diff --git a/packages/flutter_tools/lib/src/web/bootstrap.dart b/packages/flutter_tools/lib/src/web/bootstrap.dart
index 53ef4bfd3e2..5f5f6d1f697 100644
--- a/packages/flutter_tools/lib/src/web/bootstrap.dart
+++ b/packages/flutter_tools/lib/src/web/bootstrap.dart
@@ -196,6 +196,12 @@ define("$bootstrapModule", ["$entrypoint", "dart_sdk"], function(app, dart_sdk)
       return dart.getSourceMap(url);
     });
   }
+  // Prevent DDC's requireJS to interfere with modern bundling.
+  if (typeof define === 'function' && define.amd) {
+    // Preserve a copy just in case...
+    define._amd = define.amd;
+    delete define.amd;
+  }
 });
 ''';
 }
diff --git a/packages/flutter_tools/test/general.shard/web/bootstrap_test.dart b/packages/flutter_tools/test/general.shard/web/bootstrap_test.dart
index f0c28e11074..b2f3ad70569 100644
--- a/packages/flutter_tools/test/general.shard/web/bootstrap_test.dart
+++ b/packages/flutter_tools/test/general.shard/web/bootstrap_test.dart
@@ -62,6 +62,18 @@ void main() {
     expect(result, matches(regex), reason: 'require.config must have a waitSeconds: 0 config entry');
   });
 
+  test('generateMainModule hides requireJS injected by DDC', () {
+    final String result = generateMainModule(
+      entrypoint: 'foo/bar/main.js',
+      nullAssertions: false,
+      nativeNullAssertions: false,
+    );
+    expect(result, contains('''define._amd = define.amd;'''),
+      reason: 'define.amd must be preserved as _amd so users may restore it if needed.');
+    expect(result, contains('''delete define.amd;'''),
+      reason: "define.amd must be removed so packages don't attempt to use Dart's instance.");
+  });
+
   test('generateMainModule embeds urls correctly', () {
     final String result = generateMainModule(
       entrypoint: 'foo/bar/main.js',