diff --git a/packages/flutter_tools/bin/macos_assemble.sh b/packages/flutter_tools/bin/macos_assemble.sh
index 78467193681..89a00a735e6 100755
--- a/packages/flutter_tools/bin/macos_assemble.sh
+++ b/packages/flutter_tools/bin/macos_assemble.sh
@@ -191,6 +191,12 @@ EmbedFrameworks() {
   local native_assets_path="${project_path}/${FLUTTER_BUILD_DIR}/native_assets/macos/"
   if [[ -d "$native_assets_path" ]]; then
     RunCommand rsync -av --filter "- .DS_Store" --filter "- native_assets.yaml" "${native_assets_path}" "${xcode_frameworks_dir}"
+
+    # Iterate through all .frameworks in native assets directory.
+    for native_asset in "${native_assets_path}"*.framework; do
+      # Codesign the framework inside the app bundle.
+      RunCommand codesign --force --verbose --sign "${EXPANDED_CODE_SIGN_IDENTITY}" -- "${xcode_frameworks_dir}/$(basename "$native_asset")"
+    done
   fi
 }
 
diff --git a/packages/flutter_tools/lib/src/isolated/native_assets/macos/native_assets.dart b/packages/flutter_tools/lib/src/isolated/native_assets/macos/native_assets.dart
index d3428b8f732..54677a63887 100644
--- a/packages/flutter_tools/lib/src/isolated/native_assets/macos/native_assets.dart
+++ b/packages/flutter_tools/lib/src/isolated/native_assets/macos/native_assets.dart
@@ -304,7 +304,12 @@ Future<void> _copyNativeAssetsMacOS(
       ));
       await setInstallNameDylib(dylibFile);
       await createInfoPlist(name, resourcesDir);
-      await codesignDylib(codesignIdentity, buildMode, frameworkDir);
+      // Do not code-sign the libraries here with identity. Code-signing
+      // for bundled dylibs is done in `macos_assemble.sh embed` because the
+      // "Flutter Assemble" target does not have access to the signing identity.
+      if (codesignIdentity != null) {
+        await codesignDylib(codesignIdentity, buildMode, frameworkDir);
+      }
     }
     globals.logger.printTrace('Copying native assets done.');
   }
diff --git a/packages/flutter_tools/test/integration.shard/isolated/native_assets_test.dart b/packages/flutter_tools/test/integration.shard/isolated/native_assets_test.dart
index 1551663e0f5..ca79c2473a1 100644
--- a/packages/flutter_tools/test/integration.shard/isolated/native_assets_test.dart
+++ b/packages/flutter_tools/test/integration.shard/isolated/native_assets_test.dart
@@ -207,6 +207,7 @@ void main() {
           switch (buildSubcommand) {
             case 'macos':
               expectDylibIsBundledMacOS(exampleDirectory, buildMode);
+              expectDylibIsCodeSignedMacOS(exampleDirectory, buildMode);
             case 'ios':
               expectDylibIsBundledIos(exampleDirectory, buildMode);
             case 'linux':
@@ -290,6 +291,24 @@ void main() {
   }
 }
 
+void expectDylibIsCodeSignedMacOS(Directory appDirectory, String buildMode) {
+  final Directory appBundle = appDirectory.childDirectory('build/$hostOs/Build/Products/${buildMode.upperCaseFirst()}/$exampleAppName.app');
+  final Directory frameworksFolder = appBundle.childDirectory('Contents/Frameworks');
+  expect(frameworksFolder, exists);
+  const String frameworkName = packageName;
+  final Directory frameworkDir = frameworksFolder.childDirectory('$frameworkName.framework');
+  final ProcessResult codesign =
+      processManager.runSync(<String>['codesign', '-dv', frameworkDir.absolute.path]);
+  expect(codesign.exitCode, 0);
+
+  // Expect adhoc signature, but not linker-signed (which would mean no code-signing happened after linking).
+  final List<String> lines = codesign.stderr.toString().split('\n');
+  final bool isLinkerSigned = lines.any((String line) => line.contains('linker-signed'));
+  final bool isAdhoc = lines.any((String line) => line.contains('Signature=adhoc'));
+  expect(isAdhoc, isTrue);
+  expect(isLinkerSigned, isFalse);
+}
+
 /// For `flutter build` we can't easily test whether running the app works.
 /// Check that we have the dylibs in the app.
 void expectDylibIsBundledMacOS(Directory appDirectory, String buildMode) {